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KMID : 0380020090240020182
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Korean Journal of Biotechnology and Bioengineering
2009 Volume.24 No. 2 p.182 ~ p.188
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Determination of Optimal Electrotransformation Conditions for Various Lactobacillus spp
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Lee Yoo-Won
Im Sung-Hoon
Xin Chun-Feng
So Jae-Seong
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Abstract
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Lactobacillus spp., primary members of probiotics, have significant benefits for health and well-being of human. In this study Lactobacillus strains representing six species (L. paracasei KLB58, L. fermentum MS79 and KLB282, L. plantarum KLB213, L. gasseri KLB238, and L. reuteri KLB270) isolated from Korean adults were electrotransformed with plasmid pNCKH104. To determine optimal electrotransformation conditions, various conditions including cell wall weakening agent, electroporation buffer, electric field strength and time constant were tested for each strain. Overall, high transformation efficiency of approximately 2.5 5.5 CFU/ DNA was obtained where conditions of 0.5 M sucrose electroporation buffer, 1.8 kV pulse voltage and 5 ms time constant were applied. The common conditions developed in this study will make transformation of various Lactobacillus spp. easier than previous procedures.
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KEYWORD
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Lactobacillus spp, electrotransformation, optimal conditions
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